8/1/2023 0 Comments Moso bamboo![]() ![]() With the announcement of the genome sequence of Moso bamboo, it is now possible to identify and determine the molecular regulation mechanisms of all functional elements in Moso bamboo. However, there have been few molecular studies on flowering of Moso for various reasons including the difficulty of collecting tissue samples, lack of genomic knowledge, infrequent sexual reproduction and long periods of time between flowering. Several hypotheses have been proposed for the flowering mechanisms. Flowering depends on many factors, including environment, nutrition, climate and the plants' physiological status. Īs a large woody bamboo with high environmental, economic and cultural value in Asia, Moso bamboo is a perennial plant characterized by its infrequent sexual reproduction with flowering intervals ranging from several to more than a hundred years. studied the transcriptomes of developing flowers in Dendrocalamus latiflorus using Illumina sequencing. Several putative flowering-related genes have been identified from certain bamboo species –, and environmental and chemical manipulations have been found to induce bamboo flowering in vitro. Therefore, it is important to determine the specific pathways and genes involved in bamboo flowering and flower development. The reasons for this remain unclear, but it is a devastating blow to bamboo resource base. However, collective death in some bamboo species has occasionally occurred after flowering over a large area. Under normal circumstances, flowering of monopodial bamboo rarely occurs. Two types of bamboo are usually recognized – sympodial bamboos, which form clumps of culms, and monpodial bamboos which form groves. In addition, they often flower synchronously and die collectively after flowering. Woody bamboos are arborescent, perennial plants characterized by their woody stems and a prolonged vegetative phase lasting decades or even longer before flowering. Bamboos (Bambusoideae) belong to the monophyletic BEP clade (Bambusoideae, Ehrhartoideae, Pooideae) in the grass family (Poaceae), and consist of woody and herbaceous species. About 2.5 billion people depend on bamboo, ranging from food to raw materials for construction and manufacture –. Houzeau, synonym Phyllostachys heterocycla Carrière) research.īamboo is one of the most important non-timber forest products in the world. Despite its obvious potentials, this combined approach has not yet been applied to Moso bamboo ( Phyllostachys edulis (Carrière) J. With this technique, quantitative analysis of gene expression can be easily performed without potential bias, allowing a more sensitive and accurate profiling of the transcriptome. RNA-Seq analysis is powerful, enabling large-scale functional assignments of genes via the assembly of large sequenced transcriptome library. RNA-Seq refers to the whole-transcriptome shotgun sequencing of fragmented mRNA or cDNA, resulting in overlapping short reads covering the entire transcriptome. It has revolutionized biological research by rapidly providing genomic and transcriptomic data –. Next-generation sequencing (NGS) technology, such as the Illumina Solexa, Roche 454 and ABI SOLiD platforms, has emerged as a cost-effective approach for high-throughput sequencing. Transcriptome sequencing is a convenient way of rapidly obtaining information on the expressed fraction of a genome. All the authors listed have approved the manuscript that is enclosed. The corresponding author would like to declare on behalf of co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. ![]() The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have read the journal's policy and no conflict of interests exist in the submission of this manuscript, and manuscript is approved by all authors for publication. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: This work was supported by the National High Technology Research and Development Program of China “Moso Bamboo Functional Genomics Research” (grant No. Received: SeptemAccepted: Published: June 10, 2014Ĭopyright: © 2014 Gao et al. PLoS ONE 9(6):Įditor: Emmanuel Gaquerel, University of Heidelberg, Germany (2014) Characterization of the Floral Transcriptome of Moso Bamboo ( Phyllostachys edulis) at Different Flowering Developmental Stages by Transcriptome Sequencing and RNA-Seq Analysis. Citation: Gao J, Zhang Y, Zhang C, Qi F, Li X, Mu S, et al. ![]()
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